58150AST

CHIRALPAK^® AGP (5 μm) HPLC Columns

L × I.D. 10 cm × 4 mm, HPLC Column

• Synonym(s): AGP Chiral Chromatography Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: CHIRALPAK^® AGP HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4 mm
• material: stainless steel column
• Agency: suitable for USP L41
• product line: CHIRALPAK^®
• packaging: pkg of 1 ea
• manufacturer/tradename: CHIRALPAK^®
• parameter: 137 bar pressure (2000 psi); 20-30 °C temperature; 40 °C max. temp.
• technique(s): HPLC: suitable
• L × I.D.: 10 cm × 4 mm
• matrix: fully porous particle
• matrix active group: glycoprotein, alpha₁- phase
• particle size: 5 μm
• operating pH: 2-8
• separation technique: chiral

Description

General description

Hermansson described the use of natural proteins immobilized onto a silica support for chiral separations in 1983 (1). Proteins contain a large number of chiral centers of one configuration, and many other sites that contribute to the general retention process. CHIRALPAK AGP has the broadest range of selectivity of all protein phases currently available. CHIRALPAK AGP phase uses α₁-acid glycoprotein (AGP) as the chiral selector immobilized on spherical 5 μm silica particles. Bonded AGP is a very stable protein that tolerates pure organic solvents, elevated temperatures and pH values from 4 to 7. Operated in reversed-phase mode, CHIRALPAK AGP column separates enantiomers of an extremely broad range of drug substances, such as acids, amines and neutral compounds. The enantioselectivity and retention can easily be regulated by mobile phase pH and ionic strength, and the nature and concentration of the organic modifier.

Features:

• Direct reversed phase resolution of chiral molecules
• Stable with a variety of organic modifiers
• Available in analytical and semi-preparative sizes

(1) Hermansson, J. Direct liquid chromatographic resolution of racemic drugs using a1-acid glycoprotein as the chiral stationary phase. J. Chromatogr. A, 1983, 269, 71-80.

Application

• Enantiomeric methadone quantitation on real post-mortem dried matrix spots samples: Comparison of liquid chromatography and supercritical fluid chromatography coupled to mass spectrometry.: This study explores the application of enantioselective liquid chromatography techniques, including the use of CHIRALPAK^® AGP, for the quantitation of methadone enantiomers on dried matrix spots, contributing to forensic toxicology and analytical chemistry (Mueller et al., 2021).


• Enantiomeric Separation of Dioxopromethazine and its Stereoselective Pharmacokinetics in Rats by HPLC-MS/MS.: This research demonstrates the utility of chiral stationary phases, such as CHIRALPAK^® AGP, for separating enantiomers of pharmaceutical compounds, providing insights into stereoselective pharmacokinetics critical for drug development and clinical pharmacology (Lun et al., 2021).


• Evaluation of R- (-) and S- (+) Clenbuterol enantiomers during a doping cycle or continuous ingestion of contaminated meat using chiral liquid chromatography by LC-TQ-MS.: This article details the application of chiral liquid chromatography, potentially involving CHIRALPAK^® AGP columns, to distinguish between therapeutic use and contamination sources of clenbuterol, addressing challenges in sports doping analysis (Dolores et al., 2019).

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Legal Information

CHIRALPAK is a registered trademark of Daicel Corp.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable