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SAB4700062

Sigma-Aldrich

Monoclonal Anti-CD4-FITC , (N-terminal) antibody produced in mouse

clone MEM-241, purified immunoglobulin, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEM-241, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD4(920)

Related Categories

General description

Cluster of differentiation 4 (CD4) molecule is encoded by the gene mapped to human chromosome 12p13.31. The encoded protein is mainly localized on the surface of specific leukocytes. CD4 is characterized with a four ecto-domains (D1-D4) of which D1, D2 and D4 contain disulfide bonds.
The antibody MEM-241 recognizes CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes ("helper" T-cells) and also on monocytes, tissue macrophages and granulocytes.

Immunogen

2 N-terminal domains of human CD4 fused to human IgG1 Fc

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Biochem/physiol Actions

Cluster of differentiation 4 (CD4) facilitates the activation of immunostimulatory T-cells. It serves as a primary receptor for human immunodeficiency virus (HIV)-1 entry. The encoded protein also plays a key role in altering cell elasticity. Elevated expression of soluble CD4 has been observed in patients of infectious and chronic inflammatory diseases and rheumatoid arthritis (RA). Thus, this protein can be used as a potential diagnostic marker for the development of RA.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Disease severity in rheumatoid arthritis: Relationships of plasma tumor necrosis factor-α, soluble interleukin 2-receptor, soluble CD4/CD8 ratio, neopterin, and fibrin D-dimer to traditional severity and functional measures
Beckham JC, et al.
Journal of clinical immunology, 12(5), 353-361 (1992)
Human CD4 Metastability Is a Function of the Allosteric Disulfide Bond in Domain 2
Owen GR, et al.
Biochemistry, 55(15), 2227-2237 (2016)
The role of CD4 on mechanical properties of live cell membrane
Bui VC and Nguyen TH
Journal of Biomedical Materials Research Part A, 104(1), 239-244 (2016)
Isochromosomes 12p and 9p: parental origin and possible mechanisms of formation
Dutly F, et al.
European Journal of Human Genetics, 6(2), 140-144 (1998)

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