I8638
Anti-Integrin β1 antibody, Mouse monoclonal
clone W1B10, purified from hybridoma cell culture
Synonym(s):
Anti-CD29
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
purified immunoglobulin
antibody product type
primary antibodies
clone
W1B10, monoclonal
form
buffered aqueous solution
mol wt
antigen 100-140 kDa
contains
15 mM sodium azide
species reactivity
chicken
technique(s)
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 1:200 using denatured, non-reduced extract of cultured fibroblasts from chicken embryo lower leg
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
chicken ... ITGB1(374058)
General description
Monoclonal anti-β-1-Chicken Integrin (mouse IgG1 isotype) is derived from the W1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified chicken integrin from fibroblasts of 11 days old chicken embryos. Chicken integrin is a heterodimer consisting of the common β-1 subunit and two α subunits (α-5 or α-3). The β-1 subunit of chicken integrin is a 803-amino acid transmembrane glycoprotein consisting of a large extracellular domain and a short cytoplasmic domain.
Specificity
The antibody recognizes an epitope on the extracellular side of the avian integrin complex, and inhibits adhesion of embryo and tendon fibroblasts as well as skeletal myoblasts to both laminin and fibronectin. Under reducing and denaturing conditions using extracts of chicken embryo muscle cells, the antibody precipitates the β1 class integrin heterodimer as a broad band of ~140 kDa. In the absence of reducing agents, the integrin β1 subunit appears as bands at 100 kDa (immature form), 100 kDa (mature form), and 140 kDa (noncovalently associated α-subunits). The antibody may also be used for bioactivity studies in adhesion assays.
Immunogen
chicken integrin.1
Application
Anti-Integrin β1 antibody, Mouse monoclonal has been used in western blot, fluorescent activated cell sorter (FACS) analysis.
Biochem/physiol Actions
Integrin binding to extracellular matrix (ECM) ligands regulates cell adhesion, shape, motility, intracellular signalling and gene expression. The monoclonal anti-b-1 chicken integrin antibody (clone W1B10) has been used to inhibit chicken muscle cell adhesion in cell cultures.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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