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F1269

Sigma-Aldrich

Anti-Avidin–FITC antibody, Mouse monoclonal

clone WC19.10, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Avidin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

WC19.10, monoclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect immunofluorescence: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Avidin is a glycoprotein (68 kDa) composed of four identical polypeptide chains isolated from egg whites.
The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.

Specificity

FITC conjugated monoclonal anti-avidin may be used for single or double immunofluorescence labeling techniques and for modification of non-fluorescent to fluorescent reactions.

Immunogen

Purified egg white avidin.

Application

Anti-Avidin-FITC antibody, Mouse monoclonal has been used in indirect immunohistology.

Biochem/physiol Actions

Avidin has a high binding affinity for the vitamin biotin. This strong interaction has been exploited in the design of immunoassays and immunohistologic staining techniques to serve as a basis for identifying antigen antibody interactions

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 8.0, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adsorption of avidin on microfabricated surfaces for protein biochip applications
Bashir R, et al.
Biotechnology and Bioengineering, 73(4), 324-328 (2001)
John T Berg et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(8), 1101-1106 (2004-07-20)
Carbonic anhydrase (CA) is ubiquitously expressed and plays a pivotal role in acid-base balance, ion transport, and gas exchange. Limited observations by others, derived from functional, pharmacological, and histochemical studies, suggest that CA is present in vascular smooth muscle and
Xuefeng Wang et al.
Applied optics, 47(15), 2779-2789 (2008-05-22)
We perform simultaneous interferometric and fluorescent detection of molecular protein layers on a BioCD. The 488 nm excitation wavelength of fluorescein also provides the interferometric detection channel that operates in a common-path in-line configuration in the condition of phase quadrature

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