D9154

o-Dianisidine dihydrochloride

tablet

• Synonym(s): 3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B
• Empirical Formula (Hill Notation): C₁₄H₁₆N₂O₂ · 2HCl
• CAS Number: 20325-40-0
• Molecular Weight: 317.21
• NACRES: NA.83
• PubChem Substance ID: 24894263
• UNSPSC Code: 12352204
• EC Number: 243-737-5
• MDL number: MFCD00012488
• Beilstein/REAXYS Number: 3917996

Properties

• Product Name: o-Dianisidine dihydrochloride, tablet, 10 mg substrate per tablet
• Quality Segment: 200
• form: tablet
• technique(s): protein detection: suitable
• solubility: water: 1 tablet/10 mL, clear, colorless
• storage temp.: 2-8°C
• SMILES string: Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2
• InChI: 1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H
• InChI key: UXTIAFYTYOEQHV-UHFFFAOYSA-N

Description

Biochem/physiol Actions

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Preparation Note

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

Disclaimer

Probably carcinogenic.

Safety Information

• Pictograms: GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Codes: H302,H314,H350
• Precautionary Statements: P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type P3 (EN 143) respirator cartridges