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C7611

Sigma-Aldrich

Monoclonal Anti-Caspase 12 antibody produced in rat

clone 14F7, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-CASP-12, Anti-CASP12P1

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

14F7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole cell extract of rat kidney cells NRK cells

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Caspase 12 is a protein that belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The human caspase 12 gene is localized at a single locus within the caspase 1/ICE gene cluster on chromosome 11q22. The functionality is lost in humans and they cannot play a role in Alzheimer′s disease. Caspase-12 is detected in endoplasmic reticulum (ER), plays a role in ER-mediated apoptosis and inflammatory caspase activation pathway.
Monoclonal Anti-Caspase 12 (rat IgG2a isotype) is derived from the 14F7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from CD rats immunized with a recombinant mouse caspase 12. Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspase 12 is ubiquitously expressed in mouse tissues, at high levels in muscle, liver and kidney and at moderate levels in brain, where it is present in cortical neurons, Purkinje cells, brainstem neurons, and olfactory neurons.

Specificity

Recognizes full length and possibly truncated p20 caspase 12.

Immunogen

recombinant mouse caspase 12 (residues 95-318).

Application

Monoclonal Anti-Caspase 12 has been used in immunoblotting and immunocytochemistry (4% paraformaldehyde-fixed, paraffin-embedded).

Biochem/physiol Actions

Caspase 12 (CASP12) has a downregulatory function during infection and may play a role in protection against inflammatory disease. It is associated with rheumatoid arthritis in African-Americans. Caspase-12 inhibits caspase-1 activation and is associated with susceptibility factor for bacterial sepsis but does not influence the susceptibility to Candida sepsis.
Murine caspase 12 (CASP12) is activated during endoplasmic reticulum stress and activates other caspases which act on substrates, ultimately leading to apoptosis. The protein has been shown to be activated during progression of retinal degeneration in animal models. In mice models, CASP12 has been shown to cause neuronal death resulting from ischemia/reperfusion. Casp12 might stimulate nuclear factor-κB (NF-κB) by directing the post-translational degradation of inhibitor of kappa B (IκBα). Overexpression of caspase 12 induces apoptosis, which is inhibited by the pan caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethyl ketone (z-VAD-FMK).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Targeting Caspase-12 to Preserve Vision in Mice With Inherited Retinal Degeneration
Bhootada Y, et al.
Investigative Ophthalmology & Visual Science, 56(8), 4725-4725 (2015)
Caspases: evolutionary aspects of their functions in vertebrates
Sakamaki K and Satou Y
Journal of Fish Biology, 74(4), 727-753 (2009)
Differences in sustained alterations in protein expression between livers of mice exposed to high-dose-rate and low-dose-rate radiation
Nakajima T, et al.
Journal of Radiation Research, 58(4), 421-429 (2017)
Activation of caspase-12 by endoplasmic reticulum stress induced by transient middle cerebral artery occlusion in mice
Shibata M, et al.
Neuroscience, Neuroscience.(2003;118), 2-2 (2003)
Caspase 12 degrades I kappaB alpha protein and enhances MMP-9 expression in human nasopharyngeal carcinoma cell invasion
Chu WK, et al.
Testing, 8(20), 33515-33515 (2017)

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