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63535

Sigma-Aldrich

Manganese(II) chloride tetrahydrate

BioUltra, for molecular biology, ≥99.0% (KT)

Synonym(s):

Manganese chloride tetrahydrate, Manganese dichloride tetrahydrate, MnCl2.4H2O

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About This Item

Linear Formula:
MnCl2 · 4H2O
CAS Number:
Molecular Weight:
197.91
EC Number:
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

Assay

≥99.0% (KT)

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

4-6 (25 °C, 0.5 M in H2O)

mp

58 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤50 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

SMILES string

Cl[Mn]Cl.[H]O[H].[H]O[H].[H]O[H].[H]O[H]

InChI

1S/2ClH.Mn.4H2O/h2*1H;;4*1H2/q;;+2;;;;/p-2

InChI key

CNFDGXZLMLFIJV-UHFFFAOYSA-L

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Other Notes

In the tailing of DNA - MnCl2 buffer recommended for dC and dG tailing; In reverse transcription of RNA with thermostable DNA polymerase

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Oral - Eye Dam. 1 - STOT RE 2

Target Organs

Brain

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Terminal transferase: use of the tailing of DNA and for in vitro mutagenesis.
G Deng et al.
Methods in enzymology, 100, 96-116 (1983-01-01)
Nicholas Sofos et al.
Molecular cell, 79(5), 741-757 (2020-07-31)
Cmr-β is a type III-B CRISPR-Cas complex that, upon target RNA recognition, unleashes a multifaceted immune response against invading genetic elements, including single-stranded DNA (ssDNA) cleavage, cyclic oligoadenylate synthesis, and also a unique UA-specific single-stranded RNA (ssRNA) hydrolysis by the
T W Myers et al.
Biochemistry, 30(31), 7661-7666 (1991-08-06)
A recombinant DNA polymerase derived from the thermophilic eubacterium Thermus thermophilus (Tth pol) was found to possess very efficient reverse transcriptase (RT) activity in the presence of MnCl2. Many of the problems typically associated with the high degree of secondary
Lisha Luo et al.
NMR in biomedicine, 25(12), 1360-1368 (2012-05-11)
The aim of this study was to provide data on the dose dependence of manganese-enhanced MRI (MEMRI) in the visual pathway of experimental rats and to study the toxicity of MnCl₂ to the retina. Sprague-Dawley rats were intravitreally injected with
Tora Sund Morken et al.
Pediatric research, 73(2), 171-179 (2012-11-24)
Hypoxia-ischemia (HI) induces delayed inflammation and long-term gray and white matter brain injury that may be altered by hyperoxia. HI and 2 h of hyperoxia (100% O2) or room air (21% O2) in 7-d-old (P7) rats were studied by magnetic resonance

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