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antibody form
purified antibody
Quality Level
clone
1A8
monoclonal
purified by
using protein G
species reactivity
mouse, rat
concentration
(Please refer to lot specific datasheet.)
technique(s)
activity assay: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2aκ
UniProt accession no.
General description
Propeitary whole cells expressing to mouse Ly-6G.
Application
Flow Cytometry Analysis: 0.5 µg of this antibody from a representative lot detected Ly-6G in 1X10E6 mouse bone marrow cells.
Flow Cytometry Analysis: A representative lot detected Ly-6G in transfected EL4 cells and bone marrow cells (Fleming, T.J. et al. (1993). Journal of Immunology. 151(5):2399-2408).
Activity Assay Analysis: A representative lot detected Ly-6G in transmission of S. pneumoniae from index after injection with this clone (Short, K.R., et al. (2013). mBio. 3(5):e00255-12).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Ly-6G in transfected EL4 cells (Fleming, T.J. et al. (1993). Journal of Immunology. 151(5):2399-2408).
Flow Cytometry Analysis: A representative lot detected Ly-6G in transfected EL4 cells and bone marrow cells (Fleming, T.J. et al. (1993). Journal of Immunology. 151(5):2399-2408).
Activity Assay Analysis: A representative lot detected Ly-6G in transmission of S. pneumoniae from index after injection with this clone (Short, K.R., et al. (2013). mBio. 3(5):e00255-12).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Ly-6G in transfected EL4 cells (Fleming, T.J. et al. (1993). Journal of Immunology. 151(5):2399-2408).
Quality
Evaluated by Flow Cytometry in mouse splenocyte.
Flow Cytometry Analysis: 0.5 µg of this antibody detected Ly-6G in mouse splenocyte.
Flow Cytometry Analysis: 0.5 µg of this antibody detected Ly-6G in mouse splenocyte.
Physical properties
12 kDa calculated
Physical form
Purified rat monoclonal IgG2aκ in buffer containing PBS without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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