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MABC1762

Sigma-Aldrich

Anti-COPS5 Antibody

purified antibody, clone 4-9E, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

4-9E, monoclonal

mol wt

calculated mol wt 37.58 kDa
observed mol wt ~35 kDa

species reactivity

mouse, human

packaging

antibody small pack of 100 μg

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

shipped in

dry ice

storage temp.

2-8°C

target post-translational modification

unmodified

Specificity

Clone 4-9E is a mouse monoclonal antibody that detects COP9 signalosome complex subunit 5 (COPS5). It targets an epitope within the N-terminal region.

Immunogen

His-tagged recombinant fragment corresponding to the first 198 amino acids from human COP9 signalosome complex subunit 5 (COPS5).

Application

Quality Control TestingEvaluated by Western Blotting in HEK293T cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected COPS5 in HEK293T cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected COPS5 in NIH3T3 cell lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated COPS5 in Immunoprecipitation application (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).Immunohistochemistry (Paraffin) Analysis: A representative lot detected COPS5 in Immunohistochemistry application (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).Western Blotting Analysis: A representative lot detected COPS5 in Western Blotting application (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-COPS5, clone 4-9E, Cat. No. MABC1762, is a mouse monoclonal antibody that detects COPS5 and is tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Target description

COP9 signalosome complex subunit 5 (UniProt: Q92905; also known as SGN5, Signalosome subunit 5, Jun activation domain-binding protein 1, COPS5) is encoded by the COPS5 (also known as CSN5, JAB1) gene (Gene ID: 10987) in human. COPS5 is the fifth component of the COP9 signalosome (CSN) complex that directly interacts with and regulates the activity of cellular and developmental processes, including checkpoint control, DNA repair, apoptosis, and cell cycle control. This complex is an essential regulator of the ubiquitin conjugation pathway that mediates the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to a reduction in ubiquitin ligase activity of SCF-type complexes. In the CSN complex, COPS5 probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. However, it lacks the metalloproteinase activity and requires the other subunits of CSN complex. COPS5 is present both in the nucleus and cytoplasm. Its nuclear localization is reduced in the presence of interferon induced protein with tetratricopeptide repeats 3 (IFIT3). COPS5 contains a JAMM motif (aa 138-151) that is shown to be essential for the protease activity of the CSN complex. Higher levels of COPS5 are observed in a variety of human cancers and are correlated with a poor prognosis. (Ref.: Kato, J-Y., et al (2006). Hybridoma. 25(6); 342-348).

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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