MAB8126

Anti-Cytomegalovirus Antibody, late Antigen, clone 2D4.2

clone 2D4.2, Chemicon^®, from mouse

• Synonym(s): CMV
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 300
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2D4.2, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2a
• shipped in: wet ice

Description

Specificity

Reacts with a late protein M.W. 47-55 kD. Can detect CMV infection 24 hours post-infection exhibiting a cytoplasmic staining which reduces peak intensity at >72 hours.

With CMV the antigens expressed at different times are listed as:



Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

Immunogen

Epitope: late antigen

Sucrose gradient purified CMV AD169 (ATCC).

Application

Anti-Cytomegalovirus Antibody, late antigen, clone 2D4.2 detects level of Cytomegalovirus & has been published & validated for use in IF, WB, IH(P).

IFA at 1:400-1:800 on acetone fixed cells.

Works on paraffin embedded tissue sections.

Dilute with buffer pH 7.4-7.6 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.

Research Category
Infectious Diseases

Research Sub Category
Infectious Diseases - Viral

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH=7.6, 0.1% Sodium Azide.

Storage and Stability

Maintain at +2-8°C for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable