475951
MreB Perturbing Compound A22
A cell-permeable isothiourea compound that specifically, rapidly and reversibly perturbs MreB function without affecting eukaryotic actin polymerization.
Synonym(s):
MreB Perturbing Compound A22, S-(3,4-Dichlorobenzyl)isothiourea, HCl
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About This Item
Empirical Formula (Hill Notation):
C8H8Cl2N2S · xHCl
CAS Number:
Molecular Weight:
235.13 (free base basis)
MDL number:
UNSPSC Code:
51111800
NACRES:
NA.77
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Quality Level
Assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
color
white
solubility
water: 2 mg/mL
DMSO: 200 mg/mL
shipped in
ambient
storage temp.
2-8°C
InChI
1S/C8H8Cl2N2S.ClH/c9-6-2-1-5(3-7(6)10)4-13-8(11)12;/h1-3H,4H2,(H3,11,12);1H
InChI key
VBJNMXMOMSWRDV-UHFFFAOYSA-N
General description
A cell-permeable isothiourea compound that specifically, rapidly and reversibly perturbs MreB function without affecting eukaryotic actin polymerization. Reported to affect the growth and morphology of Caulobacter crescentus, convert rod-shaped E. coli to round cells, and block DNA segregation without affecting DNA replication. Suggested to interact with MreB′s nucleotide binding pocket and is useful for the temporal analysis of MreB function.
A cell-permeable isothiourea compound that specifically, rapidly, and reversibly perturbs MreB function without affecting eukaryotic actin polymerization. Reported to affect the growth and morphology of Caulobacter crescentus, convert rod-shaped E. coli to round cells, and block DNA segregation without affecting DNA replication. Suggested to interact with the nucleotide binding pocket of MreB and is useful for the temporal analysis of MreB function.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
MreB
MreB
Product does not compete with ATP.
Reversible: yes
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution in DMSO, aliquot and freeze (-20°C. DMSO stock solutions are stable for up to 1 month at -20°C. Reconstitute in H₂O just prior to use; aqueous stock solutions are stable for up to 12 hr. Stable for up to 2 days in acidic medium.
Other Notes
Gitai, Z., et al. 2005. Cell120, 329.
Jensen, R.B., et al. 2002. Nat. Rev. Mol. Cell Biol.3, 167.
Iwai, N., et al. 2002. Biosci. Biotechnol. Biochem.66, 2658.
Jensen, R.B., et al. 2002. Nat. Rev. Mol. Cell Biol.3, 167.
Iwai, N., et al. 2002. Biosci. Biotechnol. Biochem.66, 2658.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Noritaka Iwai et al.
Bioscience, biotechnology, and biochemistry, 66(12), 2658-2662 (2003-02-25)
Random screening for inhibitors of chromosome partitioning in Escherichia coli was done by the anucleate cell blue assay. A novel S-benzylisothiourea derivative, S-(3,4-dichlorobenzyl)isothiourea, tentatively named A22, was found to induce spherical cells and spherical anucleate cells in E. coli. Mecillinam
Zemer Gitai et al.
Cell, 120(3), 329-341 (2005-02-15)
Faithful chromosome segregation is an essential component of cell division in all organisms. The eukaryotic mitotic machinery uses the cytoskeleton to move specific chromosomal regions. To investigate the potential role of the actin-like MreB protein in bacterial chromosome segregation, we
Rasmus B Jensen et al.
Nature reviews. Molecular cell biology, 3(3), 167-176 (2002-05-08)
A cellular differentiation programme that culminates in an asymmetric cell division is an integral part of the cell cycle in the bacterium Caulobacter crescentus. Recent work has uncovered mechanisms that ensure the execution of many events at different times during
Abhishek Shrivastava et al.
Science advances, 6(10), eaay6616-eaay6616 (2020-03-18)
The gliding bacterium Flavobacterium johnsoniae is known to have an adhesin, SprB, that moves along the cell surface on a spiral track. Following viscous shear, cells can be tethered by the addition of an anti-SprB antibody, causing spinning at 3
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