- Immune response in guinea pigs to two different lipid conjugates of bovine serum albumin.
Immune response in guinea pigs to two different lipid conjugates of bovine serum albumin.
Journal of immunology (Baltimore, Md. : 1950) (1978-03-01)
S B Singh, S Leskowitz
PMID632585
摘要
A lipid conjugate of BSA was made by coupling dodecylamine to the COOH groups of BSA (DA-BSA). Studies on the immunogenicity of this material and one made by coupling dodecanoic anhydride to NH2 groups (D-BSA) demonstrated that cell-mediated immunity could preferentially be generated by lipid conjugation to an antigen as measured by delayed skin reaction, invitro blast transformation, and antibody formation. DA-BSA was found superior by both in vivo and in vitro tests. Both the conjugates retained the ability to suppress delayed type hypersensitivity, but only DA-BSA retained the ability to elicit optimum skin reaction in a sensitized animal and to precipitate anti-BSA antibody.