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  • Mathematical model-based optimization of physico-enzymatic hydrolysis of Pinus roxburghii needles for the production of reducing sugars.

Mathematical model-based optimization of physico-enzymatic hydrolysis of Pinus roxburghii needles for the production of reducing sugars.

Indian journal of experimental biology (2014-01-15)
Siddharth Vats, Devendra Prasad Maurya, Ayushi Jain, Varija Mall, Sangeeta Negi
摘要

The objective of this study was to optimize the physico-enzymatic pretreatment of P. roxburghii fallen foliage (needles) to produce reducing sugars through response surface methodology (RSM) with central composite face centered design (CCD). Under this, five parameters, i.e., concentration of laccase, cellulose and xylanase, steam explosion pressure and incubation period, at three levels with twenty six runs were taken into account. Cellulase, xylanase and laccase enzymes with activity 4.563, 38.32 and 0.05 IU/mL, respectively, were produced from locally isolated microbial strains. The analysis of variance (ANOVA) was applied for the validation of the predicted model at 95% of confidence level. This model predicted 334 mg/g release of reducing sugars on treating P. roxburghii fallen foliage with 1.18 mL of cellulose, 0.31 mL of xylanase and 0.01 mL of laccase, 14.39 psi steam explosion pressure and 24 h of incubation time. The experimental results obtained were in good agreement to predicted values, making it a reliable optimized model for five factors in combination to predict reducing sugar yield for ethanol production for bio-fuel industry.

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漆酶 来源于云芝, powder, light brown, ≥0.5 U/mg
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纤维素酶 来源于里氏木霉, aqueous solution, ≥700 units/g
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纤维素酶 来源于黑曲霉, powder, ≥0.3 units/mg solid
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纤维素酶 来源于曲霉 属, aqueous solution
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纤维素酶 来源于黑曲霉, powder, off-white, ~0.8 U/mg
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纤维素酶 来源于里氏木霉 ATCC 26921, lyophilized powder, ≥1 unit/mg solid
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纤维素酶 来源于木霉 属, BioReagent, suitable for plant cell culture, 3-10 units/mg solid
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纤维素酶 来源于木霉 属, powder, ≥5,000 units/g solid
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漆酶 来源于漆树, crude acetone powder, ≥50 units/mg solid
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漆酶 来源于双孢蘑菇, powder, deep brown, ≥4 U/mg
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木聚糖酶 来源于绿色木霉, lyophilized powder, 100-300 units/mg protein