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Merck

Short-term ammonium inhibition of nitrogen fixation in Azotobacter.

Biochemical and biophysical research communications (1984-09-17)
F J Cejudo, A de la Torre, A Paneque
摘要

Addition of NH4Cl at low concentrations to Azotobacter chroococcum cells caused an immediate cessation of nitrogenase activity, which was recovered once the added NH+4 was exhausted from the medium. In the presence of inhibitors of ammonium assimilation, such as L-methionine-DL-sulfoximine, L-methionine sulfone or 6-diazo-5-oxo-L-norleucine, externally added NH+4 had no effect on nitrogenase activity and the newly-fixed nitrogen was excreted into the medium as NH+4. It is concluded that, in A. chroococcum, NH+4 must be assimilated to exert its short-term inhibitory effect on nitrogen fixation.

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Sigma-Aldrich
L -蛋氨酸砜
Sigma-Aldrich
DL-蛋氨酸砜, ≥99.0% (NT)