跳转至内容
Merck
  • Effectiveness of quenchers to reduce radiolysis of (111)In- or (177)Lu-labelled methionine-containing regulatory peptides. Maintaining radiochemical purity as measured by HPLC.

Effectiveness of quenchers to reduce radiolysis of (111)In- or (177)Lu-labelled methionine-containing regulatory peptides. Maintaining radiochemical purity as measured by HPLC.

Current topics in medicinal chemistry (2013-01-24)
Erik de Blois, Ho Sze Chan, Mark Konijnenberg, Rory de Zanger, Wouter A P Breeman
摘要

An overview how to measure and to quantify radiolysis by the addition of quenchers and to maintain Radio-Chemical Purity (RCP) of vulnerable methionine-containing regulatory peptides is presented. High RCP was only achieved with a combination of quenchers. However, quantification of RCP is not standardized, and therefore comparison of radiolabelling and RCP of regulatory peptides between different HPLC-systems and between laboratories is cumbersome. Therefore we suggest a set of standardized requirements to quantify RCP by HPLC for radiolabelled DTPA- or DOTA-peptides. Moreover, a dosimetry model was developed to calculate the doses in the reaction vials during radiolabelling and storage of the radiopeptides, and to predict RCP in the presence and absence of quenchers. RCP was measured by HPLC, and a relation between radiation dose and radiolysis of RCP was established. The here described quenchers are tested individually as ƒ(concentration) to investigate efficacy to reduce radiolysis of radiolabelled methionine-containing regulatory peptides.

材料
货号
品牌
产品描述

Sigma-Aldrich
L-甲硫氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 99.0-101.0%
Sigma-Aldrich
二乙烯三胺五醋酸, ≥98% (titration)
SAFC
L-甲硫氨酸
Sigma-Aldrich
L-甲硫氨酸, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
1,4,7,10-四氮杂环十二烷-1,4,7,10-四乙酸, ≥97.0% (CHN)
Sigma-Aldrich
二乙烯三胺五醋酸, ≥99% (titration)
Sigma-Aldrich
L-甲硫氨酸, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
二乙烯三胺五醋酸, for complexometry, ≥99.0%
Supelco
L-甲硫氨酸, Pharmaceutical Secondary Standard; Certified Reference Material
蛋氨酸, European Pharmacopoeia (EP) Reference Standard
Supelco
L-甲硫氨酸, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
L-甲硫氨酸, SAJ special grade, ≥98.5%