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Merck
  • Immobilization of bacterial luciferase into poly(N-isopropylacrylamide) film for electrochemical control of a bioluminescence reaction.

Immobilization of bacterial luciferase into poly(N-isopropylacrylamide) film for electrochemical control of a bioluminescence reaction.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2012-10-13)
Yoji Kawanami, Shinya Yamasaki, Shuto Yamada, Kô Takehara
摘要

A poly(N-isopropylacrylamide) film was modified on an indium-tin oxide electrode in order to immobilize bacterial luciferase (BL) on the electrode surface. By using the modified electrode, flavin mononucleotide (FMN) was electrochemically reduced to FMNH(2), which is one of the substrates of the BL luminescence reaction, to control the bioluminescence reaction by BL. The BL reaction in the modified film could be promoted and controlled by the electrochemical generation of FMNH(2). This BL luminescence system was evaluated as a model system for the inhibitory assay of hydrophobic molecules on protein functions.

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产品描述

Sigma-Aldrich
1-十二烷醇, reagent grade, 98%
Sigma-Aldrich
1-十二烷醇, ACS reagent, ≥98.0%
Sigma-Aldrich
N -异丙基丙烯酰胺, Mn ~40,000
Sigma-Aldrich
月桂醇, ≥98%, FG
Sigma-Aldrich
羧酸封端的聚(N-异丙基丙烯酰胺), average Mn 10,000
Sigma-Aldrich
Poly(N-isopropyl acrylamide), NHS ester end functionalized, average Mn 5,000
Supelco
1-十二烷醇, Selectophore, ≥98.0%