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  • Structure of human beta 1 beta 1 alcohol dehydrogenase: catalytic effects of non-active-site substitutions.

Structure of human beta 1 beta 1 alcohol dehydrogenase: catalytic effects of non-active-site substitutions.

Proceedings of the National Academy of Sciences of the United States of America (1991-09-15)
T D Hurley, W F Bosron, J A Hamilton, L M Amzel
摘要

The three-dimensional structure of human beta 1 beta 1 alcohol dehydrogenase (ADH; EC 1.1.1.1) complexed with NAD+ has been determined by x-ray crystallography to 3.0-A resolution. The amino acids directly involved in coenzyme binding are conserved between horse EE and human beta 1 beta 1 alcohol dehydrogenase in all but one case [serine (horse) vs. threonine (human) at position 48]. As a result, the coenzyme molecule is bound in a similar manner in the two enzymes. However, the strength of the interactions in the vicinity of the pyrophosphate bridge of NAD+ appears to be enhanced in the human enzyme. Side-chain movements of Arg-47 and Asp-50 and a shift in the position of the helix comprising residues 202-212 may explain both the decreased Vmax and the decreased rate of NADH dissociation observed in the human enzyme vs. the horse enzyme. It appears that these catalytic differences are not due to substitutions of any amino acids directly involved in coenzyme binding but are the result of structural rearrangements resulting from multiple sequence differences between the two enzymes.

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Sigma-Aldrich
醇脱氢酶 来源于酿酒酵母, ≥300 units/mg protein, lyophilized powder (contains buffer salts), Mw 141-151 kDa
Sigma-Aldrich
醇脱氢酶 来源于酿酒酵母, powder, ≥300 units/mg protein, mol wt ~141,000 (four subunits)
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醇脱氢酶 来源于酿酒酵母
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乙醇脱氢酶 马, recombinant, expressed in E. coli, ≥0.5 U/mg