跳转至内容
Merck
  • The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure.

The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure.

Nature communications (2020-06-28)
Robert E Turnbull, Louise Fairall, Almutasem Saleh, Emma Kelsall, Kyle L Morris, T J Ragan, Christos G Savva, Aditya Chandru, Christopher J Millard, Olga V Makarova, Corinne J Smith, Alan M Roseman, Andrew M Fry, Shaun M Cowley, John W R Schwabe
摘要

MiDAC is one of seven distinct, large multi-protein complexes that recruit class I histone deacetylases to the genome to regulate gene expression. Despite implications of involvement in cell cycle regulation and in several cancers, surprisingly little is known about the function or structure of MiDAC. Here we show that MiDAC is important for chromosome alignment during mitosis in cancer cell lines. Mice lacking the MiDAC proteins, DNTTIP1 or MIDEAS, die with identical phenotypes during late embryogenesis due to perturbations in gene expression that result in heart malformation and haematopoietic failure. This suggests that MiDAC has an essential and unique function that cannot be compensated by other HDAC complexes. Consistent with this, the cryoEM structure of MiDAC reveals a unique and distinctive mode of assembly. Four copies of HDAC1 are positioned at the periphery with outward-facing active sites suggesting that the complex may target multiple nucleosomes implying a processive deacetylase function.

材料
货号
品牌
产品描述

Sigma-Aldrich
单克隆抗-FLAG® M2 小鼠抗, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
SKU
包装规格
是否有货
价格
数量
Sigma-Aldrich
氯仿, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
SKU
包装规格
是否有货
价格
数量
Sigma-Aldrich
抗 α-微管蛋白单克隆抗体 小鼠抗, clone DM1A, ascites fluid
SKU
包装规格
是否有货
价格
数量
Sigma-Aldrich
山羊抗兔IgG抗体,HRP偶联物, 1 mg/mL, Upstate®
SKU
包装规格
是否有货
价格
数量
Sigma-Aldrich
山羊抗小鼠IgG抗体,HRP偶联物, Upstate®, from goat
SKU
包装规格
是否有货
价格
数量
Sigma-Aldrich
驴抗山羊IgG抗体,HRP偶联,吸附物种, Chemicon®, from donkey
SKU
包装规格
是否有货
价格
数量
Sigma-Aldrich
抗HDAC2抗体,克隆3F3, clone 3F3, Upstate®, from mouse
SKU
包装规格
是否有货
价格
数量
Sigma-Aldrich
Anti-ELMSAN1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SKU
包装规格
是否有货
价格
数量
Sigma-Aldrich
p-Xylene-bis(N-pyridinium bromide), ≥95% (TLC)
SKU
包装规格
是否有货
价格
数量