Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification.

Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification.

Chemical communications (Cambridge, England) (2014-01-30)

Joel Hwang, Hai Yu, Hamed Malekan, Go Sugiarto, Yanhong Li, Jingyao Qu, Van Nguyen, Dongyuan Wu, Xi Chen

摘要

Oligo(ethylene glycol)-linked light fluorous tags have been found to be optimal for conjugating to glycans for both high-yield enzymatic glycosylation reactions using one-pot multienzyme (OPME) systems and quick product purification using fluorous solid-phase extraction (FSPE) cartridges. The combination of OPME glycosylation systems and the FSPE cartridge purification scheme provides a highly effective strategy for facile synthesis and purification of glycans.