MRC-9

85020202, human lung (fetal), Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: MRC-9, 85020202
• 生物源: human lung (fetal)
• 增長模式: Adherent
• 染色體組型: 2n = 46, diploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian foetal lung

細胞系描述

Established from normal lung tissue of a 15 week old female Caucasian foetus. Like MRC-5 the line is susceptible to a wide range of viruses. At least 40 population doublings expected.

應用

Virus studies: rhinoviruses, production of viral vaccines

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 12
D5S818: 12,13
D7S820: 10,11
THO1: 6,8
TPOX: 11
vWA: 16,17

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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