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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Supelco
Diethylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Acetone, suitable for HPLC
Sigma-Aldrich
Tetrahydrofuran, suitable for HPLC, contains no stabilizer
Sigma-Aldrich
Chloroform, suitable for HPLC
Sigma-Aldrich
Methanol, suitable for HPLC
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Methanol, JIS special grade, ≥99.8%
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Acetone, JIS special grade, ≥99.5%
Sigma-Aldrich
Sodium chloride, SAJ first grade, ≥99.0%
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Formaldehyde solution, SAJ first grade, ≥35.0%, contains methanol as stabilizer
Sigma-Aldrich
Chloroform, SAJ super special grade, ≥99.0%
Sigma-Aldrich
Methanol, SAJ first grade, ≥99.5%
Sigma-Aldrich
Sodium chloride, JIS special grade, ≥99.5%
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Piperidine, SAJ first grade, ≥99.0%
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1-Octanol, JIS special grade, ≥98.0%
Sigma-Aldrich
Sodium chloride solution, 0.1 M
Sigma-Aldrich
N,N-Dimethylformamide, JIS special grade, ≥99.5%
Sigma-Aldrich
1-Octanol, SAJ first grade, ≥75.0%
Sigma-Aldrich
Chloroform, SAJ first grade, ≥99.0%, contains 0.4-0.8% ethanol
Sigma-Aldrich
Diethyl ether, JIS special grade, ≥99.5%
Sigma-Aldrich
N,N-Dimethylformamide, SAJ first grade, ≥99.0%
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2-Mercaptoethanol, SAJ special grade, ≥99.0%
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Formaldehyde solution, JIS special grade, 36.0-38.0%, contains methanol as stabilizer
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Diethyl ether, ≥99.5%
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Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
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Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
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Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free
Sigma-Aldrich
Chloroform, ACS reagent, ≥99.8%, contains amylenes as stabilizer
Sigma-Aldrich
Diethyl ether, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
Sigma-Aldrich
N,N-Dimethylformamide, anhydrous, 99.8%
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%