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713-0141

HPLC Analysis of Barbiturates on Discovery® C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery C18, 15 cm × 4.6 mm I.D., 5 μm particles (504955)
mobile phase: [A] acetonitrile; [B] 25 mM potassium phosphate buffer, pH 6.9 (25:75, A:B)
flow rate: 1.5 mL/min
column temp.: ambient
detector: UV, 254 nm
injection: 25 μL

suitability

application for HPLC

application(s)

forensics and toxicology
pharmaceutical (small molecule)

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Methyprylon
    Hexobarbital
    Butalbital
    Butabarbital

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