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59320-U

Supelco

SUPELCOGEL™ C610 (9 µm) HPLC Columns

L × I.D. 30 cm × 7.8 mm

Synonym(s):

SUPELCOGEL™ 6% Cross Linked HPLC Columns

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Pricing and availability is not currently available.

Product Name

SUPELCOGEL™ C610H, 6% Crosslinked HPLC Column, 9 μm particle size, L × I.D. 30 cm × 7.8 mm

material

stainless steel column

Agency

suitable for USP L17

product line

SUPELCOGEL™

feature

endcapped: no

packaging

1 ea of

parameter

85 °C max. temp.

technique(s)

HPLC: suitable

L × I.D.

30 cm × 7.8 mm

matrix

sulfonated polystyrene/divinylbenzene, spherical particle platform
polymeric

particle size

9 μm

separation technique

ion exclusion

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General description

SUPELCOGEL™ C-610H, 6% crosslinked HPLC columns are prepared specifically for the analysis of organic acids. They are also used in the separation of acetic, propionic, butyric, formic, malic, citric, succinic, lactic, and other acids using a simple isocratic mobile phase and minimal sample preparation.

Legal Information

SUPELCOGEL is a trademark of Sigma-Aldrich Co. LLC

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Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Erika C Pavarina et al.
Applied biochemistry and biotechnology, 98-100, 663-677 (2002-05-23)
Four soil fungi able to grow under low oxygenation conditions were selected and used in studies to determine the production of enzymes that promote the degradation of lignocellulosic materials. The capacity of these fungi to ferment such materials was also
M Zecevic et al.
Journal of chromatography. A, 949(1-2), 61-64 (2002-05-10)
A new high-performance liquid chromatography (HPLC) method was developed for the quality control of pancuronium bromide and its degradation products. The HPLC method used a 5-microm Supelcogel ODP-50 (150x4 cm) column with acetonitrile-CH3OH-water-F3CCOOH (20.5:74.9:0.1, v/v) as the mobile phase (pH
François Becher et al.
Rapid communications in mass spectrometry : RCM, 16(6), 555-565 (2002-03-01)
There is still a need for direct determination (i.e. without dephosphorylation) of nucleoside reverse transcriptase inhibitor (NRTI) triphosphorylated nucleotides in the peripheral-blood mononuclear cells (PBMCs) of HIV-positive patients. The objective of this paper was first to improve our previously described

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Organic acid measurement complements wine quality assessment, influencing color and taste through fermentation control.

Questions

1–6 of 6 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What solutiuon can i use to clean this column?

    1 answer

    1. Cleaning Procedure:
      Metal contamination is indicated by shortened retention times and/or skewed peaks. Flush the column in the reverse flow direction following column-specific conditions listed below; do NOT connect the column to the detector during this process. Monitor the system closely to ensure that the maximum pressures are not exceeded. Note that up to 20% ethanol or up to 5% isopropanol may be substituted for the solvents recommended below.
      SUPELCOGEL K Mobile Phase: 0.1M K2HPO4 Flow: 0.1 mL/min. Temperature: 85 °C Time: 4-16 hours
      SUPELCOGEL Pb & 8 Pb Mobile Phase: 0.1M Pb(NO3)2 Flow: 0.1 mL/min. Temperature: 85 °C Time: 4-16 hours
      SUPELCOGEL Ca & 8 Ca Mobile Phase: 0.1M Ca(NO3)2 at a pH of 6.3 Flow: 0.1 mL/min. Temperature: 85 °C Time: 4-16 hours
      SUPELCOGEL H, C-610H, C-611, & 8H Mobile Phase: 10/90 CH3CN/0.01NH3PO4 Flow: 0.1 mL/min. Time: 3-4 hours at 25 °C
      SUPELCOGEL Ag1 & Ag2 No recommended cleaning procedures.

      Any additional cleaning procedures are as a last resort to prolong the column life, because the addition of organic solvents will cause the resin to swell resulting in an increase in pressure. Monitor the system closely to insure that the maximum pressures are not exceeded. To remove organic contamination, pump the columns in reverse flow at 0.1 mL/min. with 5:95 acetonitrile:water at 25 °C for 4 hours.

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. What is the % crosslinkage of the SUPELCOGEL Columns?

    1 answer

    1. All SUPELCOGEL resins are 6% crosslinked, except the SUPELCOGEL Ag2, which is 4%.

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  6. How does the Supelcogel™ H differ from the Supelcogel™ C-610H?

    1 answer

    1. Both the Supelcogel™ H and the Supelcogel™ C-610H are microparticulate, macroporous, polystyrene resin columns designed for ion exchange separation of organic acids, carbohydrates, sugar alcohols, and fermentation products.  The C-610H is manufactured with a slightly higher cross linkage.  Maximum temperature for the Supelcogel™ H is 90° C and for Supelcogel™ C-610H is 60° C.  Of the two, only the Supelcogel™ H is available in the 25cm x 4.6mm hardware.

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