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Z374911

PCR multiwell plates

size 384 wells, polypropylene, skirt, non-sterile

Synonym(s):

384 multiwell PCR plate, 384 well PCR microplate, 384 well PCR plate, 384 well microtiter plate

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About This Item

UNSPSC Code:
41106300
NACRES:
NB.22

material

colorless polypropylene
polypropylene

sterility

non-sterile
non-sterile

feature

skirt

packaging

case of 50 ea

manufacturer/tradename

Sorenson 39620

technique(s)

PCR: suitable

size

384 wells

well volume

40 μL

well working volume

25 μL

suitability

suitable for (PCR, RT-PCR or DNA purification applications)

application(s)

agriculture

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General description

384 well PCR plates are skirted for compatibility with automation systems. Wells have raised rims to ensure contact with sealing film and reduce evaporation. Each well has a capacity of 40 μL and a working volume of 25 μL.
A rigid top plate (included) minimizes plate distortion, assures a dependable fit with the PCR thermal cycler, and allows for a leak-proof seal with micro-mats or cap strips. Each well has a maximum capacity of 200 μL.

Features and Benefits

  • Virgin polypropylene
  • Fully autoclavable
  • Certified DNase- and RNase-free
  • Wells have thin walls for rapid temperature equilibration and reduced cycle time

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

dNTP Hot Start PCR Protocol

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

Primer Concentration Optimization

Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.

Reverse Transcription Protocol Using SYBR Green Dye Detection

Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.

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