This enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL, yielding a clear solution. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/134/883/x4376pis.pdf
X4376
Xanthine Oxidase from bovine milk
lyophilized powder, 0.4-1.0 units/mg protein
Synonym(s):
XOD, Xanthine:oxygen oxidoreductase
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All Photos(3)
About This Item
CAS Number:
Enzyme Commission number:
1.17.3.2.
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
biological source
bovine milk
Quality Level
form
lyophilized powder
specific activity
0.4-1.0 units/mg protein
mol wt
283 kDa
composition
Protein, 10.0-25.0%
storage temp.
2-8°C
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General description
Formerly E.C. 1.1.3.22
Application
Biochem/physiol Actions
The isoflavanoid genistein was shown to inhibit xanthine oxidase at concentrations between 0.1-4.0 μM and then activate xanthine oxidase at concentrations higher than 5 μM. [4]
Xanthine Oxidase acts as a potential biomarker for oxidative stress. It is also implicated in tissue and vascular injuries such as liver damage, inflammatory diseases and chronic heart failure.[3]
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.
Unit Definition
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Physical form
Contains 0.5% sodium salicylate.
Analysis Note
Protein determined by biuret
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Good morning, I have a question how to use Xanthine Oxidase in the form lyophilized powder? In what form can it be used? Can it be converted into a liquid form? If so, what should be the solvent? Should I use water or buffer? If a buffer witch one?
1 answer-
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