SRP4515

Artemin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

• Synonym(s): ART, ARTN, EVN, NBN
• MDL number: MFCD03099559
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 2-5 ng/mL ED₅₀ (it′s ability to stimulate the proliferation of human SH-SY5Y cells)
• mol wt: ~24 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: NM_057090
• UniProt accession no.: Q5T4W7
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... ARTN(9048)

Description

General description

Human Artemin, a neurotrophic factor, belongs to the GDNF family. Artemin is a survival factor for sensory and sympathetic neurons in culture. Recombinant human Artemin is a 24.2 kDa, disulfide-linked homodimer formed by two identical 113 amino acid subunits. The gene encoding this protein is localized on human chromosome 1p34.1.

Biochem/physiol Actions

Artemin (ART/ARTN) is a survival factor for sensory and sympathetic neurons in culture. It has a role in promoting survival of neurons. ART is also involved in the differentiation and development of the central and peripheral nervous systems. The protein promotes axonal projection and migration in sympathetic neurons. Artemin restores neural damage and diminishes neuropathic pain. It has been associated with various carcinomas.

Physical form

Lyophilized from 10 mM Sodium Citrate pH 4.5 plus 150 mM NaCl.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitution

Reconstitute in water to a concentration of 0.1-0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Recontitute in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/mL. This stock solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable