SRP2106
TATA box binding protein,GST tagged human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym(s):
GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, SCA17, TFIID
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
Recommended Products
biological source
human
recombinant
expressed in E. coli
Assay
≥70% (SDS-PAGE)
form
frozen liquid
mol wt
~64.4 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
350 μg/mL
color
clear colorless
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... TBP(6908)
Biochem/physiol Actions
TBP is the core protein of the transcription factor IID complex, the multiprotein DNA-binding factor that coordinates activities necessary for initiation of transcription by RNA polymerase II.
The TATA-binding protein (TBP) is believed to function as an essential factor of the general transcription machinery and to be involved in transcription by all three eukaryotic RNA polymerases (pol I, II, and III). TBP specifically binds to the TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor suppressor proteins.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.
A Hoffmann et al.
Nucleic acids research, 19(22), 6337-6338 (1991-11-25)
TBP, a universal eukaryotic transcription factor?
N Hernandez
Genes & development, 7(7B), 1291-1308 (1993-07-01)
M Horikoshi et al.
Cell, 54(7), 1033-1042 (1988-09-23)
The mammalian activator protein ATF stimulates transcription from the adenovirus E4 promoter by binding to multiple upstream promoter and enhancer elements. DNAase footprint analyses have revealed that there are cooperative interactions between ATF and TFIID (the mammalian TATA factor) when
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