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SHM03

Sigma-Aldrich

MISSION® ExpressMag® Beads

Increases transduction efficiency

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51
Pricing and availability is not currently available.

Quality Level

200

product line

MISSION®

shelf life

3 yr

storage temp.

2-8°C

General description

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

Application

When coupled with MISSION® ExpressMag® magnetics, ExpressMag beads will increase the transduction efficiency of lentiviral particles. Increases in transduction efficiency are particularly striking in primary cells and in suspension cells.

Preparation Note

Do NOT FREEZE! Significant decrease in efficacy will occur if ExpressMag bead solution is frozen.

Legal Information

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000284891
SLCQ7610
SLCQ8535
0000284892
SLBM2860V

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Questions

1–6 of 6 Questions  

  1. What cell lines have been transduced successfully using ExpressMag Beads?

    1 answer

    1. The following cell types have been successfully transduced using ExpressMag Beads: PBL, PBMC, T Cells, GMB primary tumor cells, A549, NYGM, T98G, U251, U87,YH-13, YK6-1, KS-1, NIH3T3, K562, Vero, HeLa, CHO, L929, B95a, H9, HUVEC, CD4+ Primary Human T-Cells, Human Bone Marrow Macrophages, Primary Keratinocytes, SKOV3, MM-AN, MEF and CD 34+.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What other viruses have been successfully introduced into cells via the use of ExpressMag beads?

    1 answer

    1. Adenovirus, SIV, Retrovirus, Measles virus and HIV have all been successfully introduced into cells using ExpressMag.

      Helpful?

  4. What MISSION products have been successfully introduced into cells using ExpressMag® beads?

    1 answer

    1. MISSION Lentiviral Particles (various targets/clones), SHC002V, SHC003V and SHC008V have been successfully introduced into cells using ExpressMag beads.

      Helpful?

  5. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  6. What is the typical fold increase in transduction efficiency when using ExpressMag beads?

    1 answer

    1. The transduction efficiency increase observed when using the ExpressMag products will depend on the cell line. It is reasonable to expect a roughly 10-fold or greater increase.

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