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SCP0088

Sigma-Aldrich

Caspase 4 Substrate (ICH-2), chromogenic

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About This Item

Empirical Formula (Hill Notation):
C28H40N6O11
Molecular Weight:
636.65
UNSPSC Code:
12352200
NACRES:
NA.32

Assay

≥95% (HPLC)

form

lyophilized

composition

Peptide Content, ≥75%

storage condition

protect from light

storage temp.

−20°C

Amino Acid Sequence

Ac-Leu-Glu-Val-Asp-pNA

Application

Caspase 4, a cysteine protease activator of inflammatory processes, may be detected using a variety of chromogenic and fluorogenic peptide substrates built around the LEVD (leu-glu-val-asp) sequence such as the chromogenic substrate Ac-LEVD-pNa (Acetyl-Leu-Glu-Val-Asp-p-nitroanalide).

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Zong-Mei Bian et al.
Investigative ophthalmology & visual science, 50(12), 6006-6014 (2009-08-01)
To investigate the functional involvement of caspase-4 in human retinal pigment epithelial (hRPE) cells. Expression and activation of caspase-4 in hRPE cells were measured after stimulation with proinflammatory agents IL-1beta (2 ng/mL), TNF-alpha (20 ng/mL), lipopolysaccharide (1000 ng/mL), interferon-gamma (500
Olga Burmistrova et al.
Cancer letters, 309(1), 71-77 (2011-06-11)
Flavonoids are naturally occurring polyphenolic compounds and are among the most promising anticancer agents. Here we demonstrate that the flavonoid derivative astragalin heptaacetate (AHA) induces cell death. This was prevented by the non-specific caspase inhibitors z-VAD-fmk and Q-VD-OPH, and reduced
Akiko Yamamuro et al.
Journal of pharmacological sciences, 115(2), 239-243 (2011-02-02)
The present study investigated the function of caspase-4 in endoplasmic reticulum (ER) stress-induced apoptosis in human neuronal cell line SH-SY5Y. Tunicamycin, which is known to induce ER stress, activated both caspase-9 and caspase-4, and the activation of caspase-4 preceded that

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