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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~29 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded human breast carcinoma indirect immunofluorescence: 0.1-0.2 μg/mL using HEK-293T cells over-expressing human ApoD western blot: 0.1-0.2 μg/mL using HEK-293T cells over-expressing human ApoD
Apoliporotein D (ApoD), a 29-kDa apolipoprotein bound to high density lipoproteins (HDL) in human plasma, is a member of the lipocalin superfamily of proteins. ApoD is widely expressed at high levels in a variety of tissues such as spleen, testes and brain (central nervous system). In the CNS, ApoD is mainly expressed by astrocytes and oligodendrocytes from late embryogenesis to aged nervous system.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminus of human apolipoprotein-D, conjugated to KLH. The corresponding sequence has 71% sequence identity in rat and mouse ApoD.
Application
Anti-ApoD (C-terminal) antibody produced in rabbit has been used in several immunochemical techniques including immunoblotting, immunofluorescence and immunohistochemistry.
Biochem/physiol Actions
Apoliporotein D (ApoD) acts as transporters of small hydrophobic ligands.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Apolipoprotein D expression in human brain reactive astrocytes
del Valle E, et al.
The Journal of Histochemistry and Cytochemistry, 51(10), 1285-1290 (2003)
Apolipoprotein D
Rassart E, et al.
Biochimica et Biophysica Acta, 1482, 185-198 (2000)
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