R6750
Ribonucleic acid from baker′s yeast (S. cerevisiae)
Synonym(s):
RNA
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About This Item
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Quality Level
storage temp.
−20°C
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General description
Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is the substrate for RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).
Application
Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.
Preparation Note
Prepared by a modification of Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
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