Ras related protein consists of three isoforms such as Rab5a, Rab5b and Rab5c. Rab5a is encoded by the gene mapped to human chromosome 3p24.3. This gene codes for 23.66 kDa Rab5a protein.
Immunogen
synthetic peptide corresponding to amino acid residues 176-188 of human Rab5 with C-terminal added cysteine, conjugated to KLH.
Application
Anti-Rab5 antibody produced in rabbit has been used in western blotting[1] and immunocytochemistry.
Biochem/physiol Actions
Rab proteins are members of small GTPases control the exocytosis and endocytosis and regulate vesicular transport. Rab5 regulates endocytic pathway in mammalian cells and an activator of autophagy. It mediates macroautophagy of cells expressing aggregates of mutant Huntingtin protein.
Rab5a is essential for epidermal growth factor (EGF)-mediated EGF receptor degradation. Overexpression of Rab5a is associated with the development of variety of cancers such as breast cancer, lung cancer, hepatocellular carcinoma (HCC) and ovarian cancer.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Endosomal trafficking of cell surface receptors is essential to their function. Integrins are transmembrane receptors that integrate adhesion to the extracellular matrix with engagement of the cytoskeleton. Ligated integrins mediate diverse signals that regulate matrix assembly, cell survival, cell morphology
Journal of cell science, 113 Pt 2, 183-192 (2000-01-14)
Endocytosis is characterized by vesicular transport along numerous pathways. Common steps in each pathway include membrane budding to form vesicles, transport to a particular destination, and ultimately docking and fusion with the target membrane. Specificity of vesicle targeting is rendered
Lysosomal network proteins as potential novel CSF biomarkers for Alzheimer?s disease
Armstrong A, et al.
Neuromolecular Medicine, 16(1), 150-160 (2014)
Rab5a promotes the migration and invasion of hepatocellular carcinoma by up-regulating Cdc42
Yang X, et al.
International Journal of Clinical and Experimental Pathology, 11(1), 224-231 (2018)
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