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Key Documents

MAK369

Sigma-Aldrich

Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric)

sufficient for 200 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

neurological disorders; cardiovascular diseases; cancer

General description

Antioxidants are molecules which act as reducing agents by donating electrons to free radicals to stabilize them and minimize the damage caused by free radicals to DNA, cells and organ systems. Antioxidants include substances such as polyphenols; flavonoids; vitamins and enzymes like glutathione peroxidase and superoxide dismutase. They are known to have beneficial health effects such as lowering the risk of cancer, heart disease and neurodegenerative disorders and are abundantly found in plants, fruits, vegetables, beverages and natural products.

Application


  • Biochemical Characterization of Different Chemical Components of Parthenium hysterophorus and Their Therapeutic Potential against HIV-1 RT and Microbial Growth.: This research article examines the chemical components of Parthenium hysterophorus, utilizing the Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric) to assess their antioxidant capabilities. The study highlights the therapeutic potential of these components against HIV-1 reverse transcriptase (RT) and microbial growth, offering new insights into the medicinal properties of the plant (Jaiswal et al., 2022).


Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of antioxidant capacity in fruits, vegetables, beverages (like tea and wine), food products, plant extracts, herbal products, serum and plasma.

Principle

Ferric reducing antioxidant power (FRAP) assay is a widely used method that uses antioxidants as reductants in a redox-linked colorimetric reaction, wherein Fe3+ is reduced to Fe2+. Ferric (Fe3+) to ferrous (Fe2+) ion reduction at low pH causes formation of a colored ferrous-probe complex from a colorless ferric-probe complex. The assay is high-throughput adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C


Certificates of Analysis (COA)

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