Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

MAK117

Sigma-Aldrich

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Synonym(s):

Glycerin Assay Kit, Glycerine Assay Kit

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 200 colorimetric or fluorometric tests

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−20°C

General description

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations[1] and is the main waste by-product of biodiesel production via transesterification.

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples[2] and to measure plasma glycerol levels.[3]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Principle

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
117CE09A13
117CE03A27
117CE03A04
117CE03A04
117CE03A27

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Rodrigo Fernández-Verdejo et al.
Obesity (Silver Spring, Md.), 28(6), 1110-1116 (2020-05-06)
This study aimed to determine the relationship between metabolic flexibility (MetFlex) measured during a euglycemic-hyperinsulinemic clamp and a prolonged fast. This study also analyzed the association between MetFlex and metabolic health. Eighteen healthy men (mean [SD]: 22 [2] years old;
Nicolae S.
Health & Drugs: Disease, Prescription & Medication (2014)
Meichun Zhang et al.
PLoS neglected tropical diseases, 13(2), e0007139-e0007139 (2019-02-05)
As an important vector of dengue and Zika, Aedes albopictus has been the fastest spreading invasive mosquitoes in the world over the last 3-4 decades. Cold tolerance is important for survival and expansion of insects. Ae. albopictus adults are generally
Jessica C Brooks et al.
The Analyst, 141(20), 5714-5721 (2016-08-04)
Employing 3D-printed templates for macro-to-micro interfacing, a passively operated polydimethysiloxane (PDMS) microfluidic device was designed for time-resolved secretion sampling from primary murine islets and epidiymal white adipose tissue explants. Interfacing in similar devices is typically accomplished through manually punched or
Martina Rudnicki et al.
Frontiers in physiology, 9, 1452-1452 (2018-11-09)
Background: Impaired capillary growth (angiogenesis) in skeletal muscle and adipose tissue contributes to the development of metabolic disorders in obese males. This association remains unexplored in females, despite mounting evidence that endothelial cells have sex-specific transcriptional profiles. Therefore, herein we
View All Related Papers

Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. Is there any information available on how to prepare tissue for assay, considering that technical bulletin MAK117 does not include any recommendations on this matter?

    1 answer

    1. The general recommendation for tissue sample preparation is as follows:
      1. Begin with 20-100 mg tissue and add 200-1000 uL ice-cold PBS.
      2. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice-water bath). The degree of tissue lysis can be checked under a microscope.
      3. Centrifuge the homogenate at 14,000 g for 10 min.
      4. Transfer the clear supernatant into a clean tube and use it for the assay.

      Helpful?

  3. Can serum glycerol content be analyzed or just plasma?

    1 answer

    1. Yes, this product can determine glycerol content in serum samples.

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/409/mak117bul.pdf

      Helpful?

  4. How long are reagents stable at 2-8 C after initial thaw?

    1 answer

    1. The Assay Buffer, MAK117A, may be stored at 2–8 Celsius. For all other components, it is recommended to aliquot and freeze at - 20 deg C. Avoid repleated freeze/thaw cycles.

      Helpful?

  5. How is shipping temperature determined? And how is it related to the product storage temperature?

    0 answers

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service