F4125
Ficin from fig tree latex
saline suspension, ≥1.0 units/mg protein (biuret)
Synonym(s):
Debricin, Ficain, higueroxyl delabarre
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
fig tree (latex)
form
saline suspension
specific activity
≥1.0 units/mg protein (biuret)
mol wt
23.8 kDa
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
General description
Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Application
Ficin from fig tree latex has been used:
- in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality
- for the digestion of eye tissue sections prior to immunostaining and immunofluorescence
Biochem/physiol Actions
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.
Unit Definition
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).
Physical form
Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0
Preparation Note
2× Crystallized
inhibitor
Product No.
Description
Pricing
substrate
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex
Zare H, et al.
Phytochemistry, 87, 16-22 (2013)
Transgenic expression of leukemia inhibitory factor (LIF) blocks normal vascular development but not pathological neovascularization in the eye
Ash J, et al.
Molecular Vision, 11, 298-308 (2005)
Yufang Yang et al.
Scientific reports, 7, 43141-43141 (2017-02-23)
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for
Lens-specific VEGF-A expression induces angioblast migration and proliferation and stimulates angiogenic remodeling
Ash JD and Overbeek PA
Developmental Biology, 223(2), 383-398 (2000)
Kristien Bonroy et al.
Journal of immunological methods, 312(1-2), 167-181 (2006-05-06)
The sensitivity of immunosensors is strongly dependent on the amount of immobilised antibodies and their remaining antigen binding properties. The use of smaller and well-oriented antibody fragments as bioreceptor molecules influences the final immunosensor signal. The aim of this study
Protocols
This procedure may be used for all Ficin products.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service