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D8938

Sigma-Aldrich

KIMBLE Dounce tissue grinder set

KIMBLE Dounce tissue grinder set

2 mL complete

Synonym(s):

885300-0002, Dounce, Dounce Homogenizer, Glass Tissue Grinder, KIMBLE Tissue Grinder, KONTES, Pestles, Tissue Grinder, Tubes

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About This Item

UNSPSC Code:
41121800
NACRES:
NB.22
Pricing and availability is not currently available.

material

glass

feature

autoclavable

manufacturer/tradename

Kimble® 885300-0002

pestle A clearance

0.0030-0.0050 in.

pestle B clearance

0.0005-0.0025 in.

working volume × L

2 mL × 60 mm

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General description

Designed primarily for cellular work where the nucleus remains intact after homogenization. All-glass construction. Two pestles are supplied with each complete set. Large clearance pestle is used for the initial sample reduction. Small clearance pestle is used to form the final homogenate.

Features and Benefits

• Manufactured from borosilicate glass 3.3• Designed primarily for cellular work where the nucleus remains intact after homogenization.• All-glass construction• Two pestles are supplied with each complete unit• Large clearance pestle is used for the initial sample reduction• Small clearance pestle is used to form the final homogenate• Replacement components are available and completely interchangeable

Legal Information

KIMBLE is a registered trademark of DWK Life Sciences

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Fang-Yuan Hu et al.
Frontiers in bioengineering and biotechnology, 8, 564057-564057 (2020-10-20)
Retina is a crucial tissue for capturing and processing light stimulus. It is critical to describe the characteristics of retina at the single-cell level for understanding its biological functions. A variety of abnormalities in terms of morphology and function are
Naomi Habib et al.
Nature neuroscience, 23(6), 701-706 (2020-04-29)
The role of non-neuronal cells in Alzheimer's disease progression has not been fully elucidated. Using single-nucleus RNA sequencing, we identified a population of disease-associated astrocytes in an Alzheimer's disease mouse model. These disease-associated astrocytes appeared at early disease stages and
Naomi Habib et al.
Nature methods, 14(10), 955-958 (2017-08-29)
Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human
Benjamin C Reiner et al.
G3 (Bethesda, Md.), 10(5), 1647-1655 (2020-03-07)
Long interspersed element-1 retrotransposons (LINE-1 or L1) are ∼6 kb mobile DNA elements implicated in the origins of many Mendelian and complex diseases. The actively retrotransposing L1s are mostly limited to the L1 human specific (L1Hs) transcriptional active (Ta) subfamily.
Nur Jury et al.
Clinical epigenetics, 12(1), 32-32 (2020-02-20)
Hexanucleotide repeat expansions of the G4C2 motif in a non-coding region of the C9ORF72 gene are the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Tissues from C9ALS/FTD patients and from mouse models of ALS

Questions

1–2 of 2 Questions  
  1. Is it possible to purchase individual pestles for the D8938 KIMBLE Dounce tissue grinder set?

    Is it possible to purchase individual pestles for the D8938 KIMBLE Dounce tissue grinder set?

    1 answer
    1. This product is only sold as a set. You may want to check with the manufacturer to inquire if the pestles can be obtained separately. For further information, you can contact them at the following link: https://www.dwk.com/na/contact-us. The Kimble catalog number for this item is Kimble® 885300-0002.

      Helpful?

  2. Can we use this double homogeniser for mitochondria isolation from cells?

    1 answer
    1. This product has not been validated for use in mitochondrial isolation. This is an application that will need to be determined by the end-user, however there are a number of publications citing this product in organelle isolation. It may be helpful to review page 12 of the following journal article:
      https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/febs.14028

      It also may be helpful to view the peer-reviewed articles:
      https://www.sigmaaldrich.com/search/d8938?focus=papers&page=1&perpage=30&sort=relevance&term=d8938&type=citation_search

      Helpful?

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