D4138
Deoxyribonuclease II from porcine spleen
Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)
Synonym(s):
DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Pricing and availability is not currently available.
Recommended Products
type
Type IV
Quality Level
form
lyophilized powder
specific activity
2,000-6,000 Kunitz units/mg protein (biuret)
mol wt
45 kDa
composition
Protein, 60-80%
foreign activity
RNase ≤0.05%
storage temp.
−20°C
Application
DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase.[1] It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).[2]
Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.
Biochem/physiol Actions
Unit Definition
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM
Physical form
Contains sodium chloride
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Yoko Kato-Toma et al.
The Biochemical journal, 371(Pt 1), 175-181 (2003-01-07)
13C-NMR spectroscopy was used to estimate the p K a values for the Tyr(150) (Y150) residue in wild-type and mutant class C beta-lactamases. The tyrosine residues of the wild-type and mutant lactamases were replaced with (13)C-labelled L-tyrosine ([ phenol -4-(13)C]tyrosine)
U Plöckinger et al.
European journal of endocrinology, 166(2), 223-234 (2011-11-09)
Somatostatin analogues (SSA) reduce autonomous GH secretion by activating somatostatin receptors (sst) 2 and 5 in 50-60% of acromegalic patients. However, by inhibiting insulin secretion these SSA reduce glucose tolerance. DG3173 is a novel SSA with additional binding to sst4
F Chianini et al.
Veterinary immunology and immunopathology, 82(3-4), 245-255 (2001-10-06)
The distribution of different cells of the immune system has been studied in formalin-fixed paraffin-embedded tissues from conventionally reared healthy pigs, using immunohistological techniques. The samples collected were: lungs, tonsils, lymph nodes (mediastinal, mesenteric, inguinal and submandibular), pancreas, spleen, liver
C C Wang et al.
The Journal of biological chemistry, 273(27), 17192-17198 (1998-06-27)
Porcine spleen DNase II, a lysosomal acid hydrolase, is a noncovalently linked alpha.beta heterodimer (Liao, T.-H. (1985) J. Biol. Chem. 260, 10708-10713). The alpha subunit, after disulfide cleavage, yields two chains, alpha1 and alpha2. The complete amino acid sequences of
T H Liao et al.
Biochimica et biophysica acta, 1007(1), 15-22 (1989-01-23)
Porcine spleen DNase II (EC 3.1.22.1), one of the best-characterized DNases II, is subcellularly located in lysosomes because the enzyme is co-sedimented with two of the lysosomal marker enzymes, cathepsin D and acid phosphatase. The physicochemical properties, including the subunit
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
