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D2189

Sigma-Aldrich

Recombinant CRM197 expressed in Pseudomonas fluorescens, [Glu52]- Diphtheria toxin

lyophilized powder

Synonym(s):

[Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, CRM 197

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About This Item

CAS Number:
UNSPSC Code:
12352202
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form

lyophilized powder

Quality Level

storage temp.

2-8°C

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Packaging

Package size based on protein content

Physical form

Lyophilized powder containing sodium phosphate, sucrose and polysorbate 80

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Lucia H Lee et al.
Clinical and vaccine immunology : CVI, 19(4), 551-556 (2012-02-18)
New multivalent CRM(197)-based conjugate vaccines are available for childhood immunization. Clinical studies were reviewed to assess meningococcal group C (MenC) antibody responses following MenC-CRM(197) coadministration with CRM(197)-based pneumococcal or Haemophilus influenzae type b conjugate vaccines. Infants receiving a total CRM(197)
Matthias A Oberli et al.
Chemistry & biology, 18(5), 580-588 (2011-05-26)
Nosocomial infections with the Gram-positive pathogen Clostridium difficile pose a major risk for hospitalized patients and result in significant costs to health care systems. Here, we present the chemical synthesis of a PS-II hapten of a cell wall polysaccharide of
Yung-Chih Kuo et al.
Colloids and surfaces. B, Biointerfaces, 91, 242-249 (2011-12-06)
This study investigates the capability of CRM197-grafted polybutylcyanoacrylate (PBCA) nanoparticles (NPs) (CRM197/PBCA NPs) to carry zidovudine (AZT) across the blood-brain barrier (BBB). AZT was loaded on CRM197/PBCA NPs to traverse the monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by
Dodi Safari et al.
Vaccine, 29(38), 6498-6504 (2011-07-20)
Memory formation to CRM-neoglycoconjugate, a synthetic branched tetrasaccharide of Streptococcus pneumoniae type 14 polysaccharide (Pn14PS) that is conjugated to a CRM197 protein, was investigated using mice models. Mice were first immunized with the CRM-neoglycoconjugate and then boosted with either the
Pierre van Damme et al.
PloS one, 6(9), e25398-e25398 (2011-10-08)
Typhoid fever causes more than 21 million cases of disease and 200,000 deaths yearly worldwide, with more than 90% of the disease burden being reported from Asia. Epidemiological data show high disease incidence in young children and suggest that immunization

Questions

1–4 of 4 Questions  
  1. How do I reconstitute Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?

    1 answer
    1. The vial can be reconstituted with 1.0 mL of sterile water. Each vial contains 1 mg of Diphtheria toxin CRM mutant, sodium phosphate buffer, and lactose. It can sometimes be hard to get the product into solution due to the lactose used as a stabilizer. The supplier suggests slight warming (to at least room temperature or 30 °C, but not more than 37 °C) followed by a brief vortex to break up the filaments.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. What is the molecular weight of Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?

    1 answer
    1. On reducing SDS gels, this protein migrates as a single band of an approximate molecular weight of 63,000 Daltons.

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  4. What kind of mutant, is Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, and how is this product different from Product No. D0564?

    1 answer
    1. Product No. D0564 is the wild-type toxin and possesses enzymatic activity.  The Glu52 diptheria toxin, also known as CRM197, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity. More information on the mutant can be found in the following reference: Uchida, T., Pappenheimer, Jr., A.M. and Harper, A.A.,  J. Biol. Chem., 248, 3851-3854 (1973).

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