D0547
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DME/F-12, 1:1 mixture
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About This Item
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Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
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General description
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:
- to culture cerebral cortices from mice embryos
- as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
- to culture human corneal epithelial cell lines
Other Notes
Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate
Quantity
Formulated to contain 12.0 grams of powder per liter of medium.
Reconstitution
Supplement with 1.2 g/L sodium bicarbonate
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Nutrient Mixture F-12 Ham Formulation
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