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U-87 MG Cell Line human

89081402, human brain (glioblastoma astrocytoma), Epithelial-like

Synonym(s):

U-87MG Cells, U87 Cells, U87MG Cells

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About This Item

UNSPSC Code:
41106514
biological source:
human brain (glioblastoma astrocytoma)
growth mode:
Adherent
karyotype:
2n = 46
morphology:
Epithelial-like
products:
Not specified
receptors:
Not specified
relevant disease(s):
cancer
Pricing and availability is not currently available.

Product Name

U-87 MG Cell Line human, from human brain(glioblastoma astrocytoma), 89081402

biological source

human brain (glioblastoma astrocytoma)

growth mode

Adherent

karyotype

2n = 46

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human glioblastoma astrocytoma

Cell Line Description

Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.

Application

Neural studies
U-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma.[1] It has also been used to study calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields.[2]

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. je voudrai connaitre le nombre de passage des deux lignées Pour la déclaration codecoh, j'ai besoin de votre numéro de DC ou AC ou IE, Merci

    1 answer

    1. The passage number for ECACC cell lines is lot specific and reported on the Certificate of Analysis. This document may be accessed directly at the supplier's website. Please see the link below to review the ECCAC product page:
      https://www.culturecollections.org.uk/nop/product/u-87-mg

      Helpful?

  2. Quante cellule per batch?

    1 answer

    1. This supplier cell bank, ECACC, states that adherent/attached cell line products are 2 - 3 x10^6 cells per vial, which would be the case for this product. Suspension cell lines from this supplier are 4 - 5 x10^6 cells per vial. They do not share 'batch' size information. For additional information please see the ECACC product page at the link : https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=89081402&collection=ecacc_gc

      Helpful?

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