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Clone C6/36

89051705, insect larvae, Not specified

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About This Item

UNSPSC Code:
41106514

product name

Clone C6/36, 89051705

biological source

insect larvae

growth mode

Adherent

karyotype

Diploid

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mosquito larvae, Aedes albopictus

Cell Line Description

Derived from Aedes albopictus cells. Useful for propagation of Dengue virus and flaviviruses. Cells are non-anchorage dependant, not tumourigenic and maintain a diploid chromosome number.

Application

Clone C6/36 has been used to screen anti-Wolbachia compounds. It has also been used to study the effects of tick-borne encephalitis virus (TBEV) vaccine.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Sodium Bicarbonate (NaHCO3) level of 1.1g/l is recommended).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 5000 - 30000 cells/cm2 . Cells are dislodged by mechanical agitation; 5% CO2; 28°C. Freeze in 10% glycerol.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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