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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0/U
Reactivity
Anti-Macrophage subset, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS0/U x Mouse BALB/c spleen
Cell Line Description
The antibody recognises a rat macrophage cell surface antigen and labels all peritoneal and 90% of alveolar macrophages. Splenic red pulp macrophages are antigen +ve, marginal zone macrophages antigen -ve. The antibody also reacts with the 120kDa antigen on granulocytes and dendritic cells and recognises rat SIRP (personal communication).
Immunogen
Resident peritoneal cells from (PVG-RT1c x PVG RT1u)F1 and (PVG-RT1c x DA-RT1a)F1 rats
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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Certificates of Analysis (COA)
Lot/Batch Number
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