utr-1
Mouse monoclonal against human CD-120b, 10080904
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About This Item
UNSPSC Code:
41106514
biological source
Human x Mouse Hybridoma
ImmunoDon
Mouse (Mus musculus) spleen
Myeloma
PAI
Reactivity
Human CD120b (TNF receptor p75)
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Application
Sandwich ELISA for soluble p75 TNF-R. No competition with TNF.
Culture Medium
Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 25 U/ml Interleukin-6 (IL-6) + 5-10% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA may be required to dislodge them from the plastic.
Subculture Routine
Seed cultures between 1-3 x 105 cells/ml; Saturation density 8-10 x 105 5% CO2; 37 °C
Other Notes
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Certificates of Analysis (COA)
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