UM-RC-3

8090512, human kidney (renal gland), Polygonal

• UNSPSC Code: 41106514

Properties

• product name: UM-RC-3, 08090512
• biological source: human kidney (renal gland)
• growth mode: Adherent
• karyotype: Median number of chromosomes is 55
• morphology: Polygonal
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human renal carcinoma

Cell Line Description

UM-RC-3 is a continuous cell line established from a primary renal adenocarcinoma. It exhibits a loss of contact inhibition and produces tumours in the nude mouse model. The patient had metastatic disease at the time of their nephrectomy.

Application

Study of tumour associated antigens.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9,12
D16S539: 9
D5S818: 9,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Doubling time approximately 29 hours.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.