108CC15

8062516, Mouse x Rat Hybridoma nerve, Neuronal

• UNSPSC Code: 41106514

Properties

• product name: 108CC15, 08062516
• biological source: Mouse x Rat Hybridoma nerve
• growth mode: Adherent
• karyotype: Modal No. varied with passage see reference
• morphology: Neuronal
• products: Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
• receptors: Receptors for neurohormones: delta opioid, PGE1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse neuroblastoma x Rat glioma hybrid

Cell Line Description

The cell line108CC15 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-15 by Klee and Nirenberg. Sigma has two catalogue entries for the 108CC15 cell line which differ in that they were deposited by different groups: 108CC15 from the original group that derived the cell line (Sigma Catalogue number 08062516) and NG108-15 (Sigma Catalogue number 88112302). Several other cell lines in the Sigma catalogue have been derived from 108CC15. This is a cholinergic hybrid cell line.

Application

Used as a neuronal Model

Culture Medium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

Other Notes

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