C3400

Casein from bovine milk

powder

• CAS Number: 9000-71-9
• EC Number: 232-555-1
• MDL number: MFCD00081481
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: bovine milk
• Quality Level: 200
• Assay: 87-94% protein basis
• form: powder
• technique(s): activity assay: suitable; electrophoresis: suitable; immunocytochemistry: suitable
• mp: 280 °C (dec.) (lit.)
• solubility: H₂O: insoluble (forms a cloudy suspension)
• UniProt accession no.: P02662; P02663; P02666; P02668
• InChI: 1S/C81H125N22O39P/c1-36(2)31-50(76(132)94-43(15-24-57(87)108)71(127)101-52(34-64(120)121)78(134)98-49(81(137)138)11-7-8-30-82)99-72(128)47(19-28-61(114)115)95-77(133)51(33-63(118)119)100-73(129)48(20-29-62(116)117)97-80(136)65(37(3)104)103-75(131)44(16-25-58(88)109)92-68(124)42(14-23-56(86)107)90-67(123)41(13-22-55(85)106)91-69(125)45(17-26-59(110)111)93-70(126)46(18-27-60(112)113)96-79(135)53(35-142-143(139,140)141)102-74(130)40(12-21-54(84)105)89-66(122)39(83)32-38-9-5-4-6-10-38/h4-6,9-10,36-37,39-53,65,104H,7-8,11-35,82-83H2,1-3H3,(H2,84,105)(H2,85,106)(H2,86,107)(H2,87,108)(H2,88,109)(H,89,122)(H,90,123)(H,91,125)(H,92,124)(H,93,126)(H,94,132)(H,95,133)(H,96,135)(H,97,136)(H,98,134)(H,99,128)(H,100,129)(H,101,127)(H,102,130)(H,103,131)(H,110,111)(H,112,113)(H,114,115)(H,116,117)(H,118,119)(H,120,121)(H,137,138)(H2,139,140,141)
• InChI key: BECPQYXYKAMYBN-UHFFFAOYSA-N
• Gene Information: bovine ... CSN1S1(282208) , CSN1S2(282209) , CSN2(281099) , CSN3(281728)

Description

General description

Casein from bovine milk is a phosphoprotein and forms three-dimensional colloidal supramolecular micelles. There are four main types of casein which make up approximately 80% of the total protein in bovine milk: α-s1 casein, α-s2 casein, β-casein, and κ-casein. Casein is proposed to be the main protective constituent in milk. Casein is an amphiphilic protein.

Application

Casein from bovine milk has been used:

• as a solid food sample in the in vivo and the in vitro digestion experiments using rodents
• to prepare P407-casein hydrogels and to study the mechanical effects of the addition of casein to P407
• in the preincubation solution, to increase the photostability of the quantum dots and to decrease nonspecific binding, for real-time imaging of single synaptic vesicles in hippocampal neurons

Casein from bovine milk is a phosphoprotein. There are four main types of Casein which make up approximately 80% of the total protein in bovine milk: α-s1 Casein, α-s2 Casein, β-Casein, and κ-Casein. Casein is proposed to be the main protective constituent in milk.

Biochem/physiol Actions

Casein is useful in food industries and non-food applications. It has the property for emulsification, foam formation, and stabilization, water-binding, and gelation. Casein is also considered heat and acid stable. It can serve as an indispensable diet for rodents.

Partial gastrointestinal digestion of casein is a rich source of bioactive peptides, such as β-casomorphin. However, bovine casein is not homogeneous; variants A1 and B do lead to production of β-casomorphin 7 production, while A2 does not.

Quality

Essentially vitamin free.

Preparation Note

Lactic acid precipitated New Zealand casein extracted with ethyl alcohol.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves