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14202

Sigma-Aldrich

Diisopropyl-fluorophosphatase

≥30 U/mg

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About This Item

CAS Number:
Enzyme Commission number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

fermentation/recombinant

form

lyophilized solid

specific activity

≥30 U/mg

storage temp.

−20°C

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

One unit corresponds to the amount of enzyme which hydrolyzes 1 μmol diisopropyl fluorophosphate per minute at pH 8.1 and 22 °C

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marc-Michael Blum et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 63(Pt 1), 42-45 (2006-12-22)
The enzyme diisopropyl fluorophosphatase (DFPase) from Loligo vulgaris is capable of decontaminating a wide variety of toxic organophosphorus nerve agents. DFPase is structurally related to a number of enzymes, such as the medically important paraoxonase (PON). In order to investigate
Marc-Michael Blum et al.
Journal of the American Chemical Society, 128(39), 12750-12757 (2006-09-28)
A wide range of organophosphorus nerve agents, including Soman, Sarin, and Tabun is efficiently hydrolyzed by the phosphotriesterase enzyme diisopropyl fluorophosphatase (DFPase) from Loligo vulgaris. To date, the lack of available inhibitors of DFPase has limited studies on its mechanism.
Paul D Adams et al.
Acta crystallographica. Section D, Biological crystallography, 65(Pt 6), 567-573 (2009-05-26)
X-ray and neutron crystallographic techniques provide complementary information on the structure and function of biological macromolecules. X-ray and neutron (XN) crystallographic data have been combined in a joint structure-refinement procedure that has been developed using recent advances in modern computational
Dragomir I Draganov
Chemico-biological interactions, 187(1-3), 370-372 (2010-02-04)
Serum paraoxonase (PON1) is well recognized for its ability to hydrolyze arylesters, toxic oxon metabolites of organophosphate insecticides and nerve agents. PON1 is a member of gene family including also PON2 and PON3; however, the later two enzymes have very
T Wille et al.
Toxicology in vitro : an international journal published in association with BIBRA, 24(3), 1026-1031 (2009-12-08)
Developing improved medical countermeasures against chemical warfare agents (nerve agents) is urgently needed but time-consuming and costly. Here we introduce a robot-assisted liquid handling system with warming, cooling and incubating facilities to screen the detoxifying properties of biological and chemical

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