For staining dinoflagellates using Neutral Red powder, it is recommended to use the same basic formulation as the Neutral Red solution offered as catalog number N2889, which is stated to be 3.3 g/L dissolved in a DPBS. It should be possible to dissolve in any suitable cell culture media that does not contain phenol red in the formulation. Neutral Red is a vital stain, so the dye needs to be dissolved into an isotonic solution and should not be dissolved in water.
861251
Neutral Red
certified by the Biological Stain Commission
Synonym(s):
3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
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About This Item
Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
Molecular Weight:
288.78
Colour Index Number:
50040
Beilstein:
3918740
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Recommended Products
grade
certified by the Biological Stain Commission
Quality Level
form
powder
composition
Dye content, 50-60%
technique(s)
titration: suitable
pH
6.8-8.0, red to yellow
mp
290 °C (dec.) (lit.)
solubility
water: 1 mg/mL
λmax
540 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
InChI
1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
InChI key
PGSADBUBUOPOJS-UHFFFAOYSA-N
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Application
Neutral red has been used as a staining agent for micro- and meso-plankton.[1] It has been used as a staining agent for nematocytes.[2]
Useful as an indicator for preparing neutral red paper, and as a biological stain.
Biochem/physiol Actions
Neutral red is useful as an indicator for preparing neutral red paper, and as a biological stain. Neutral dye has low toxicity and is a vital dye. It can also be used as a pH indicator.[3] It is used for cell viability assay. The dye accumulates in the lysosomes of living healthy cells, but not in damaged cells.[4]
Suitability
Certified for use in the method of Doan & Ralph for the supravital staining of living blood cells.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Customers Also Viewed
The Anatomical Record, 27, 241-241 (1924)
Cell Growth, Differentiaton and Senescence (1999)
Dead or alive? Viability assessment of micro-and mesoplankton.
Zetsche EV and Meysman FJ
Journal of Plankton Research, 34, 493-509 (2012)
PH and Brain Function (1998)
Clung,C.E., Ed.
Handbook of Microscopical Technique, 571-571 (1950)
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How to prepare Neutral Red 861251 for staining dinoflagellates?
1 answer-
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What percentage is this neutral red solution?
1 answer-
The dye content specification for this product is 50 - 60% Neutral Red. The exact percentage is lot-specific and will be reported on the Certificate of Analysis. See the link below for a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIAL/861251/11714LEHelpful?
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