51558

HEPES buffered saline

BioUltra, for molecular biology, 2× concentrate

• Synonym(s): HEPES Saline Buffer Solution
• MDL number: MFCD00006158
• UNSPSC Code: 12161700
• PubChem Substance ID: 329757705
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioUltra
• form: liquid
• quality: 2× concentrate
• composition: dextrose, 2.0 g/L ; HEPES, 10 g/L ; KCl, 0.74 g/L ; NaCl, 16 g/L ; Na₂HPO₄.2H₂O, 0.27 g/L
• concentration: 2 ×
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.339
• pH: 7.2 (25 °C)
• cation traces: Al: ≤1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.06; λ: 280 nm A_max: 0.04
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES buffered saline is a tissue culture buffer,[1] which is used to maintain the pH of cell culture.[2] It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.[3]

Application

HEPES buffered saline has been used:

• for the preparation of plasmid DNA[4][5]
• as a reagent to prepare transfection master mix for DNA construct vectors[6]
• to develop hydrogel precursor for cell leakage assay[7]

Components

concentration (in H₂O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na₂HPO₄.2H₂O; 2.0 g/l dextrose

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves