ERT-RO
Roche
Expand™ Reverse Transcriptase
solution, recombinant, expressed in E. coli (AP401 (k)), sufficient for 20 reactions, sufficient for 100 reactions
Synonym(s):
reverse transcriptase, expand
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About This Item
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recombinant
expressed in E. coli (AP401 (k))
form
solution
usage
sufficient for 100 reactions
sufficient for 20 reactions
packaging
pkg of 1,000 U (11785826001 [20 μl])
pkg of 5,000 U (11785834001 [100 μl])
manufacturer/tradename
Roche
parameter
42-43 °C optimum reaction temp.
technique(s)
RT-PCR: suitable
RT-qPCR: suitable
input
purified RNA
detection method
probe-based
storage temp.
−20°C
General description
Expand™ Reverse Transcriptase is a RNA directed DNA polymerase optimized for amplification of cDNA fragments up to 13.5 kb in a two-step RT-PCR assay. The enzyme is a genetically engineered version of the Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT). A point mutation within the RNase H sequence reduces RNase H activity to undetectable levels. Detectable improvements are obtained with respect to higher amounts of full-length cDNA transcripts and longer transcripts compared to using the native M-MuLV-RT.
Specificity
Heat inactivation: 95 °C for 2 min
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Application
Expand™ Reverse Transcriptase has been used in real-time PCR analysis for conversion of isolated RNA into cDNA. It has also been used in reverse transcription (RT). Expand™ Reverse Transcriptase may also be used for analyzing the sequence of the cDNA and comparing it to the genomic sequence. Important information can be obtained on intervening sequences (introns) and genomic recombinations.
Features and Benefits
- Obtain higher amounts of full-length cDNA transcripts and longer transcripts up to 13.5kb
- No RNase H activity
- Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
- Ideal for two-Step RT-PCR assay
- Purification of cDNA before PCR reaction is not necessary
Packaging
1 set containing 3 components
Unit Definition
One unit is the enzyme activity that incorporates 1.0 nmol TMP into acid insoluble products in 10 min at +37 °C with poly(A)+ × (dT)15 as substrate.
Volume Activity: 50 U/μl
Volume Activity: 50 U/μl
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
Expand is a trademark of Roche
Kit Components Only
Product No.
Description
- Expand Reverse Transcriptase 50 U/μl
- Expand Reverse Transcriptase Buffer 5x concentrated
- DTT Stock Solution 100 mM
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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